Description: Rabbit polyclonal antibody to PRAS40 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PRAS40. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: AKT1S1 Alternative Names: PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrateEntrez Gene (Human): 84335Entrez Gene (Mouse) : 67605SwissProt (Human): Q96B36SwissProt (Mouse) : Q9D1F4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PRAS40 expression in HepG2 (A), EC9706 (B), rat kidney (C) whole cell lysates.
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  Immunohistochemical analysis of PRAS40 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.