Description: Rabbit polyclonal antibody to Alpha-adducin (Phospho-T445) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T445 of human Alpha-adducin protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ADD1 Alternative Names: ADDA; Alpha-adducin; Erythrocyte adducin subunit alphaEntrez Gene (Human): 118Entrez Gene (Mouse) : 11518Entrez Gene (Rat) : 24170SwissProt (Human): P35611SwissProt (Mouse) : Q9QYC0SwissProt (Rat) : Q63028Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Alpha-adducin (Phospho-T445) expression in mouse liver (A), rat liver (B) whole cell lysates.
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Immunohistochemical analysis of Alpha-adducin (Phospho-T445) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.