Description: Rabbit polyclonal antibody to MARCH1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MARCH1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MARCH1 Alternative Names: RNF171; E3 ubiquitin-protein ligase MARCH1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; MARCH-I; RING finger protein 171Entrez Gene (Human): 55016Entrez Gene (Mouse) : 72925SwissProt (Human): Q8TCQ1SwissProt (Mouse) : Q6NZQ8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MARCH1 expression in Raw264.7 (A) whole cell lysates.
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Immunohistochemical analysis of MARCH1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.