Anti-ZIC1/2/3 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to ZIC1/2/3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIC1/2/3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: ZIC1; ZIC2; ZIC3 Alternative Names: ZIC1; ZIC; ZNF201; Zinc finger protein ZIC 1; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZIC2; Zinc finger protein ZIC 2; Zinc finger protein of the cerebellum 2; ZIC3; ZNF203; Zinc finger protein ZIC 3; Zinc finger protein 203; Zinc finger protein of the cerebellum 3Entrez Gene (Human): 7545; 7546; 7547Entrez Gene (Mouse) : 22771; 22773SwissProt (Human): Q15915; O95409; O60481SwissProt (Mouse) : P46684; Q62520; Q62521Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ZIC1/2/3 expression in SW480 (A), Jurkat (B), SHSY5Y (C) whole cell lysates. -
Immunohistochemical analysis of ZIC1/2/3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of ZIC1/2/3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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