Description: Rabbit polyclonal antibody to ZIC1/2/3/4/5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIC1/2/3/4/5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: ZIC1 Alternative Names: ZIC1; ZIC; ZNF201; Zinc finger protein ZIC 1; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZIC2; Zinc finger protein ZIC 2; Zinc finger protein of the cerebellum 2; ZIC3; ZNF203; Zinc finger protein ZIC 3; Zinc finger protein 203; Zinc finger protein of the cerebellum 3; ZIC4; Zinc finger protein ZIC 4; Zinc finger protein of the cerebellum 4; ZIC5; Zinc finger protein ZIC 5; Zinc finger protein of the cerebellum 5Entrez Gene (Human): 7545; 7546; 7547; 84107; 85416Entrez Gene (Mouse) : 22771; 22773; 22774SwissProt (Human): Q15915; O95409; O60481; Q8N9L1; Q96T25SwissProt (Mouse) : P46684; Q62520; Q62521; Q61467Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of ZIC1/2/3/4/5 expression in rat brain (A) whole cell lysates.
• 
  Immunofluorescent analysis of ZIC1/2/3/4/5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.