Description: Rabbit polyclonal antibody to WNK1 (Phospho-T60) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T60 of human WNK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: WNK1 Alternative Names: HSN2; KDP; KIAA0344; PRKWNK1; Serine/threonine-protein kinase WNK1; Erythrocyte 65 kDa protein; p65; Kinase deficient protein; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; hWNK1Entrez Gene (Human): 65125Entrez Gene (Mouse) : 232341Entrez Gene (Rat) : 116477SwissProt (Human): Q9H4A3SwissProt (Mouse) : P83741SwissProt (Rat) : Q9JIH7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of WNK1 (Phospho-T60) expression in HEK293T EGF-treated (A), Jurkat EGF-treated (B) whole cell lysates.
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  Immunohistochemical analysis of WNK1 (Phospho-T60) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.