Description: Rabbit polyclonal antibody to UBA5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human UBA5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: UBA5 Alternative Names: UBE1DC1; Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1Entrez Gene (Human): 79876Entrez Gene (Mouse) : 66663Entrez Gene (Rat) : 300968SwissProt (Human): Q9GZZ9SwissProt (Mouse) : Q8VE47SwissProt (Rat) : Q5M7A4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UBA5 expression in C6 (A), CT26 (B), A549 (C), HepG2 (D), HCT116 (E) whole cell lysates.
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  Immunohistochemical analysis of UBA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.