Description: Rabbit polyclonal antibody to TTF1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TTF1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TTF1 Alternative Names: Transcription termination factor 1; TTF-1; RNA polymerase I termination factor; Transcription termination factor I; TTF-IEntrez Gene (Human): 7270Entrez Gene (Mouse) : 22130SwissProt (Human): Q15361SwissProt (Mouse) : Q62187Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TTF1 expression in HEK293T (A) whole cell lysates.
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Immunohistochemical analysis of TTF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.