Description: Rabbit polyclonal antibody to TSC2 (Phospho-S939) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S939 of human TSC2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TSC2 Alternative Names: TSC4; Tuberin; Tuberous sclerosis 2 proteinEntrez Gene (Human): 7249Entrez Gene (Rat) : 24855SwissProt (Human): P49815SwissProt (Mouse) : Q61037SwissProt (Rat) : P49816Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TSC2 (Phospho-S939) expression in HEK293T insulin-treated (A), NIH3T3 insulin-treated (B) whole cell lysates.
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  Immunohistochemical analysis of TSC2 (Phospho-S939) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.