Description: Rabbit polyclonal antibody to TRIM38 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRIM38. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: TRIM38 Alternative Names: RNF15; RORET; Tripartite motif-containing protein 38; RING finger protein 15; Zinc finger protein RoRetEntrez Gene (Human): 10475SwissProt (Human): O00635Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TRIM38 expression in SGC7901 (A), HEK293T (B), A549 (C), mouse embryo (D), rat stomach (E), H9C2 (F) whole cell lysates.
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Immunohistochemical analysis of TRIM38 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
E3 ubiquitin ligase tripartite motif 38 negatively regulates TLR-mediated immune responses by proteasomal degradation of TNF receptor-associated factor 6 in macrophages