Description: Rabbit polyclonal antibody to CARK Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CARK. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TNNI3K Alternative Names: CARK; Serine/threonine-protein kinase TNNI3K; Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinaseEntrez Gene (Human): 100526835; 51086Entrez Gene (Mouse) : 435766Entrez Gene (Rat) : 295531SwissProt (Human): Q59H18SwissProt (Mouse) : Q5GIG6SwissProt (Rat) : Q7TQP6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CARK expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
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  Immunohistochemical analysis of CARK staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.