Description: Rabbit polyclonal antibody to CD252 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD252. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: TNFSF4 Alternative Names: TXGP1; Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; CD252Entrez Gene (Human): 7292Entrez Gene (Mouse) : 22164Entrez Gene (Rat) : 89814SwissProt (Human): P23510SwissProt (Mouse) : P43488SwissProt (Rat) : Q9Z2P3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD252 expression in mouse spleen (A), HEK293T (B) whole cell lysates.
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Immunohistochemical analysis of CD252 staining in human lymph formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.