Description: Rabbit polyclonal antibody to CD253 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD253. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: TNFSF10 Alternative Names: APO2L; TRAIL; Tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD253Entrez Gene (Human): 8743Entrez Gene (Mouse) : 22035SwissProt (Human): P50591SwissProt (Mouse) : P50592Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD253 expression in HEK293T (A), Hela (B), H460 (C), mouse kidney (D), mouse testis (E), rat kidney (F), rat testis (G) whole cell lysates.
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  Immunohistochemical analysis of CD253 staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.