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Anti-TCF4/12 Antibody CPA4275
  • Western blot analysis of TCF4/12 expression in H446 (A), mouse liver (B) whole cell lysates.
  • Immunohistochemical analysis of TCF4/12 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-TCF4/12 Antibody
Cat No: CPA4275
Source: Rabbit
Reactivity: H, M, R, B, C, P
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to TCF4/12
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TCF4/12. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol: TCF4; TCF12
Alternative Names: TCF4; BHLHB19; ITF2; SEF2; Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2; TCF12; BHLHB20; HEB; HTF4; Transcription factor 12; TCF-12; Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4
Entrez Gene (Human): 6925; 6938
Entrez Gene (Mouse) : 21413; 21406
Entrez Gene (Rat) : 84382; 25720
SwissProt (Human): P15884; Q99081
SwissProt (Mouse) : Q60722; Q61286
SwissProt (Rat) : Q62655; P51514
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of TCF4/12 expression in H446 (A), mouse liver (B) whole cell lysates.
  • Immunohistochemical analysis of TCF4/12 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Inactivation of a Single Copy of Crebbp Selectively Alters Pre-mRNA Processing in Mouse Hematopoietic Stem Cells
Journal PLoS One
IF 3.7
Application WB
Reactivity Mouse
PMID 21901164
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