Description: Rabbit polyclonal antibody to SRF (Phospho-S103) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S103 of human SRF protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), EMSA (Use at an assay dependent dilution) Gene Symbol: SRF Alternative Names: Serum response factor; SRFEntrez Gene (Human): 6722Entrez Gene (Mouse) : 20807SwissProt (Human): P11831SwissProt (Mouse) : Q9JM73Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SRF (Phospho-S103) expression in HeLa (A), Jurkat (B) whole cell lysates.
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Immunohistochemical analysis of SRF (Phospho-S103) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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