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Anti-SRF (Phospho-S103) Antibody CPA4257
  • Western blot analysis of SRF (Phospho-S103) expression in HeLa (A), Jurkat (B) whole cell lysates.
  • Immunohistochemical analysis of SRF (Phospho-S103) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-SRF (Phospho-S103) Antibody
Cat No: CPA4257
Source: Rabbit
Reactivity: H, M
Applications: WB, IH, EMSA
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$420
100 μl
$260
30 μl
$130
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Description: Rabbit polyclonal antibody to SRF (Phospho-S103)
Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S103 of human SRF protein. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), EMSA (Use at an assay dependent dilution)
Gene Symbol: SRF
Alternative Names: Serum response factor; SRF
Entrez Gene (Human): 6722
Entrez Gene (Mouse) : 20807
SwissProt (Human): P11831
SwissProt (Mouse) : Q9JM73
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of SRF (Phospho-S103) expression in HeLa (A), Jurkat (B) whole cell lysates.
  • Immunohistochemical analysis of SRF (Phospho-S103) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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IF
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Reactivity
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