Description: Rabbit polyclonal antibody to SPINK6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SPINK6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SPINK6 Alternative Names: Serine protease inhibitor Kazal-type 6; Kallikrein inhibitorEntrez Gene (Human): 404203Entrez Gene (Mouse) : 433180Entrez Gene (Rat) : 100911369; 408235SwissProt (Human): Q6UWN8SwissProt (Mouse) : Q8BT20SwissProt (Rat) : Q6IE47Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SPINK6 expression in HeLa (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
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Immunohistochemical analysis of SPINK6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.