Anti-Alpha-synuclein (Phospho-S129) Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to Alpha-synuclein (Phospho-S129) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S129 of human Alpha-synuclein protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: SNCA Alternative Names: NACP; PARK1; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACPEntrez Gene (Human): 6622Entrez Gene (Mouse) : 20617Entrez Gene (Rat) : 29219SwissProt (Human): P37840SwissProt (Mouse) : O55042SwissProt (Rat) : P37377Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Alpha-synuclein (Phospho-S129) expression in HEK293T (A), mouse brain (B) whole cell lysates. -
Immunohistochemical analysis of Alpha-synuclein (Phospho-S129) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of Alpha-synuclein (Phospho-S129) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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