Description: Rabbit polyclonal antibody to Smoothened Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Smoothened. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SMO Alternative Names: SMOH; Smoothened homolog; SMO; Protein GxEntrez Gene (Human): 6608Entrez Gene (Mouse) : 319757SwissProt (Human): Q99835SwissProt (Mouse) : P56726Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Smoothened expression in HeLa (A) whole cell lysates.
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  Immunohistochemical analysis of Smoothened staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.