Description: Rabbit polyclonal antibody to SMIT1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SMIT1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SLC5A3 Alternative Names: Sodium/myo-inositol cotransporter; Na(+)/myo-inositol cotransporter; Sodium/myo-inositol transporter 1; SMIT1; Solute carrier family 5 member 3Entrez Gene (Human): 6526Entrez Gene (Mouse) : 53881SwissProt (Human): P53794SwissProt (Mouse) : Q9JKZ2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SMIT1 expression in human prostate (A), rat kidney (B) whole cell lysates.
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Immunohistochemical analysis of SMIT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.