Description: Rabbit polyclonal antibody to CARKL Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CARKL. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SHPK Alternative Names: CARKL; Sedoheptulokinase; SHK; Carbohydrate kinase-like proteinEntrez Gene (Human): 23729SwissProt (Human): Q9UHJ6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CARKL expression in human brain (A) whole cell lysates.
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Immunohistochemical analysis of CARKL staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism