Anti-SGK1 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to SGK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SGK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: SGK1 Alternative Names: SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1Entrez Gene (Human): 6446Entrez Gene (Mouse) : 20393SwissProt (Human): O00141SwissProt (Mouse) : Q9WVC6SwissProt (Rat) : Q06226Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SGK1 expression in Jurkat (A), HepG2 (B) whole cell lysates. -
Immunohistochemical analysis of SGK1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of SGK1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse prefrontal cortex
Role for the kinase SGK1 in stress, depression, and glucocorticoid effects on hippocampal neurogenesis
The dissection of transcriptional modules regulated by various drugs of abuse in the mouse striatum
Mechanisms for acute stress-induced enhancement of glutamatergic transmission and working memory
Rescue of DeltaF508-CFTR by the SGK1/Nedd4-2 signaling pathway