Description: Rabbit polyclonal antibody to SDCCAG8 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SDCCAG8. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SDCCAG8 Alternative Names: CCCAP; NPHP10; Serologically defined colon cancer antigen 8; Antigen NY-CO-8; Centrosomal colon cancer autoantigen protein; hCCCAPEntrez Gene (Human): 10806Entrez Gene (Mouse) : 76816SwissProt (Human): Q86SQ7SwissProt (Mouse) : Q80UF4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SDCCAG8 expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
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Immunohistochemical analysis of SDCCAG8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.