Description: Rabbit polyclonal antibody to Nogo Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Nogo. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RTN4 Alternative Names: KIAA0886; NOGO; Reticulon-4; Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5Entrez Gene (Human): 57142Entrez Gene (Mouse) : 68585Entrez Gene (Rat) : 83765SwissProt (Human): Q9NQC3SwissProt (Mouse) : Q99P72SwissProt (Rat) : Q9JK11Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Nogo expression in mouse brain (A), rat brain (B), K562 (C), U87MG (D) whole cell lysates.
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Immunohistochemical analysis of Nogo staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.