Description: Rabbit polyclonal antibody to RAD52B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD52B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RDM1 Alternative Names: RAD52B; RAD52 motif-containing protein 1; RAD52 homolog BEntrez Gene (Human): 201299Entrez Gene (Mouse) : 66599SwissProt (Human): Q8NG50SwissProt (Mouse) : Q9CQK3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of RAD52B expression in mouse testis (A), rat testis (B) whole cell lysates.
-
Immunohistochemical analysis of RAD52B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.