Description: Rabbit polyclonal antibody to G15 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human G15. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RBM5 Alternative Names: RNA-binding protein 5; Protein G15; Putative tumor suppressor LUCA15; RNA-binding motif protein 5; Renal carcinoma antigen NY-REN-9Entrez Gene (Human): 10181Entrez Gene (Mouse) : 83486Entrez Gene (Rat) : 300996SwissProt (Human): P52756SwissProt (Mouse) : Q91YE7SwissProt (Rat) : B2GV05Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of G15 expression in K562 (A), Jurkat (B) whole cell lysates.
-
Immunohistochemical analysis of G15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay