Description: Rabbit polyclonal antibody to PTP1B (Phospho-S50) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S50 of human PTP1B protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PTPN1 Alternative Names: PTP1B; Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1BEntrez Gene (Human): 5770Entrez Gene (Mouse) : 19246Entrez Gene (Rat) : 24697SwissProt (Human): P18031SwissProt (Mouse) : P35821SwissProt (Rat) : P20417Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PTP1B (Phospho-S50) expression in zebrafish (A) whole cell lysates.
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Immunohistochemical analysis of PTP1B (Phospho-S50) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.