Description: Rabbit polyclonal antibody to Prothymosin alpha Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Prothymosin alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PTMA Alternative Names: TMSA; Prothymosin alphaEntrez Gene (Human): 5757Entrez Gene (Mouse) : 19231Entrez Gene (Rat) : 100359583; 29222SwissProt (Human): P06454SwissProt (Mouse) : P26350SwissProt (Rat) : P06302Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Prothymosin alpha expression in U87MG (A), Panc1 (B), HCT116 (C), rat testis (D), mouse brain (E) whole cell lysates.
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Immunohistochemical analysis of Prothymosin alpha staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.