Description: Rabbit polyclonal antibody to Protein C Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Protein C. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PROC Alternative Names: Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIVEntrez Gene (Human): 5624Entrez Gene (Mouse) : 19123Entrez Gene (Rat) : 25268SwissProt (Human): P04070SwissProt (Mouse) : P33587SwissProt (Rat) : P31394Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Protein C expression in Raw264.7 (A), HepG2 (B), Jurkat (C), HEK293T (D) whole cell lysates.
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Immunohistochemical analysis of Protein C staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.