Description: Rabbit polyclonal antibody to PRKD2 (Phospho-S876) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S876 of human PRKD2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PRKD2 Alternative Names: PKD2; Serine/threonine-protein kinase D2; nPKC-D2Entrez Gene (Human): 25865SwissProt (Human): Q9BZL6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PRKD2 (Phospho-S876) expression in HEK293T (A), HEK293T-PMA-5 min (B) whole cell lysates.
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Immunohistochemical analysis of PRKD2 (Phospho-S876) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Protein Kinase D1 Mediates Stimulation of DNA Synthesis and Proliferation in Intestinal Epithelial IEC-18 Cells and in Mouse Intestinal Crypts