Description: Rabbit polyclonal antibody to DARPP32 (Phospho-T75) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T75 of human DARPP32 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PPP1R1B Alternative Names: DARPP32; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoproteinEntrez Gene (Human): 84152Entrez Gene (Mouse) : 19049Entrez Gene (Rat) : 360616SwissProt (Human): Q9UD71SwissProt (Mouse) : Q60829SwissProt (Rat) : Q6J4I0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DARPP32 (Phospho-T75) expression in mouse lung (A), mouse liver (B), rat lung (C), rat liver (D) whole cell lysates.
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  Immunohistochemical analysis of DARPP32 (Phospho-T75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.