Description: Rabbit polyclonal antibody to DNA Polymerase gamma 2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DNA Polymerase gamma 2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: POLG2 Alternative Names: MTPOLB; DNA polymerase subunit gamma-2 mitochondrial; DNA polymerase gamma accessory 55 kDa subunit; p55; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-betaEntrez Gene (Human): 11232Entrez Gene (Mouse) : 50776SwissProt (Human): Q9UHN1SwissProt (Mouse) : Q9QZM2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DNA Polymerase gamma 2 expression in rat kidney (A) whole cell lysates.
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Immunohistochemical analysis of DNA Polymerase gamma 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.