Description: Rabbit polyclonal antibody to Phospholamban (Phospho-S16/T17) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S16/T17 of human Phospholamban protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: PLN Alternative Names: PLB; Cardiac phospholamban; PLBEntrez Gene (Human): 5350Entrez Gene (Mouse) : 18821Entrez Gene (Rat) : 64672SwissProt (Human): P26678SwissProt (Mouse) : P61014SwissProt (Rat) : P61016Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Phospholamban (Phospho-S16/T17) expression in human heart (A) whole cell lysates.
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Immunofluorescent analysis of Phospholamban (Phospho-S16/T17) staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation