Description: Rabbit polyclonal antibody to Phospholipase D4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Phospholipase D4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PLD4 Alternative Names: C14orf175; Phospholipase D4; PLD 4; Choline phosphatase 4; Phosphatidylcholine-hydrolyzing phospholipase D4Entrez Gene (Human): 122618Entrez Gene (Mouse) : 104759SwissProt (Human): Q96BZ4SwissProt (Mouse) : Q8BG07Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Phospholipase D4 expression in PC12 (A), CT26 (B), Hela (C), A549 (D) whole cell lysates.
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  Immunohistochemical analysis of Phospholipase D4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.