Description: Rabbit polyclonal antibody to PDHA1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PDHA1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PDHA1 Alternative Names: PHE1A; Pyruvate dehydrogenase E1 component subunit alpha, somatic form mitochondrial; PDHE1-A type IEntrez Gene (Human): 5160Entrez Gene (Mouse) : 18597SwissProt (Human): P08559SwissProt (Mouse) : P35486SwissProt (Rat) : P26284Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PDHA1 expression in HepG2 (A) whole cell lysates.
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  Immunohistochemical analysis of PDHA1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.