Description: Rabbit polyclonal antibody to PDCD4 (Phospho-S457) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S457 of human PDCD4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PDCD4 Alternative Names: H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15aEntrez Gene (Human): 27250Entrez Gene (Mouse) : 18569Entrez Gene (Rat) : 64031SwissProt (Human): Q53EL6SwissProt (Mouse) : Q61823SwissProt (Rat) : Q9JID1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PDCD4 (Phospho-S457) expression in HeLa (A), Jurkat (B) whole cell lysates.
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  Immunohistochemical analysis of PDCD4 (Phospho-S457) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.