Description: Rabbit polyclonal antibody to PCK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PCK1 Alternative Names: PEPCK1; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK-CEntrez Gene (Human): 5105Entrez Gene (Mouse) : 18534Entrez Gene (Rat) : 362282SwissProt (Human): P35558SwissProt (Mouse) : Q9Z2V4SwissProt (Rat) : P07379Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PCK1 expression in H446 (A), rat kidney (B), rat testis (C) whole cell lysates.
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Immunohistochemical analysis of PCK1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
In vitro, in vivo, and in silico evaluation of the glucocorticoid receptor antagonist activity of 3, 6-dibromocarbazole