Anti-NOTCH1 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to NOTCH1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NOTCH1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: NOTCH1 Alternative Names: TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1Entrez Gene (Human): 4851Entrez Gene (Mouse) : 18128Entrez Gene (Rat) : 25496SwissProt (Human): P46531SwissProt (Mouse) : Q01705SwissProt (Rat) : Q07008Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NOTCH1 expression in Myla2059 (A) whole cell lysates. -
Immunohistochemical analysis of NOTCH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of NOTCH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
The jagged-2/notch-1/hes-1 pathway is involved in intestinal epithelium regeneration after intestinal ischemia-reperfusion injury
Downregulation of microRNA-30 facilitates podocyte injury and is prevented by glucocorticoids
Gene expression and functional annotation of the human ciliary body epithelia
Inhibition of Fibroblast Growth by Notch1 Signaling Is Mediated by Induction of Wnt11-Dependent WISP-1
Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders
Preventive effect of Notch signaling inhibition by a gamma-secretase inhibitor on peritoneal dialysis fluid-induced peritoneal fibrosis in rats