Description: Rabbit polyclonal antibody to NDUFA3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NDUFA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NDUFA3 Alternative Names: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; Complex I-B9; CI-B9; NADH-ubiquinone oxidoreductase B9 subunitEntrez Gene (Human): 4696Entrez Gene (Mouse) : 66091SwissProt (Human): O95167SwissProt (Mouse) : Q9CQ91Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NDUFA3 expression in mouse heart (A), rat heart (B) whole cell lysates.
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Immunohistochemical analysis of NDUFA3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.