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Anti-p47 phox (Phospho-S304) Antibody CPA4109
  • Western blot analysis of p47 phox (Phospho-S304) expression in HEK293T (A), Hela (B), mouse lung (C), mouse liver (D), rat liver (E), rat spleen (F) whole cell lysates.
  • Immunohistochemical analysis of p47 phox (Phospho-S304) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-p47 phox (Phospho-S304) Antibody
Cat No: CPA4109
Source: Rabbit
Reactivity: H, M, R, B, D, S
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$420
100 μl
$260
30 μl
$130
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Description: Rabbit polyclonal antibody to p47 phox (Phospho-S304)
Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S304 of human p47 phox protein. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol: NCF1
Alternative Names: NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox
Entrez Gene (Human): 653361
SwissProt (Human): P14598
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of p47 phox (Phospho-S304) expression in HEK293T (A), Hela (B), mouse lung (C), mouse liver (D), rat liver (E), rat spleen (F) whole cell lysates.
  • Immunohistochemical analysis of p47 phox (Phospho-S304) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: Role of PKC?-mediated p47phox phosphorylation
Journal Chem Biol Interact
IF 5.1
Application WB
Reactivity Human
PMID 20920494
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