Description: Rabbit polyclonal antibody to MIA1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MIA1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MIA Alternative Names: Melanoma-derived growth regulatory protein; Melanoma inhibitory activity proteinEntrez Gene (Human): 8190Entrez Gene (Mouse) : 12587Entrez Gene (Rat) : 81510SwissProt (Human): Q16674SwissProt (Mouse) : Q61865SwissProt (Rat) : Q62946Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MIA1 expression in HeLa (A), Raw264.7 (B), H9C2 (C) whole cell lysates.
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  Immunohistochemical analysis of MIA1 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.