Description: Rabbit polyclonal antibody to MEKK4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MEKK4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MAP3K4 Alternative Names: KIAA0213; MAPKKK4; MEKK4; MTK1; Mitogen-activated protein kinase kinase kinase 4; MAP three kinase 1; MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4Entrez Gene (Human): 4216Entrez Gene (Mouse) : 26407SwissProt (Human): Q9Y6R4SwissProt (Mouse) : O08648Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MEKK4 expression in A549 (A), EC9706 (B) whole cell lysates.
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Immunohistochemical analysis of MEKK4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.