Description: Rabbit polyclonal antibody to CD229 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD229. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: LY9 Alternative Names: T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD229Entrez Gene (Human): 4063Entrez Gene (Mouse) : 17085SwissProt (Human): Q9HBG7SwissProt (Mouse) : Q01965Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD229 expression in SGC7901 (A), HCT116 (B), HUT78 (C) whole cell lysates.
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  Immunohistochemical analysis of CD229 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.