Anti-JUNB Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to JUNB Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human JUNB. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) Gene Symbol: JUNB Alternative Names: Transcription factor jun-BEntrez Gene (Human): 3726Entrez Gene (Mouse) : 16477Entrez Gene (Rat) : 24517SwissProt (Human): P17275SwissProt (Mouse) : P09450SwissProt (Rat) : P24898Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of JUNB expression in MCF7 (A), Raw264.7 (B), PC12 (C) whole cell lysates. -
Immunohistochemical analysis of JUNB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Immunofluorescent analysis of JUNB staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1 pathway
JunB transcription factor maintains skeletal muscle mass and promotes hypertrophy
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Identifying intrinsic and extrinsic determinants that regulate internal initiation of translation mediated by the FMR1 5' leader