Description: Rabbit polyclonal antibody to IRX3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IRX3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: IRX3 Alternative Names: IRXB1; Iroquois-class homeodomain protein IRX-3; Homeodomain protein IRXB1; Iroquois homeobox protein 3Entrez Gene (Human): 79191Entrez Gene (Mouse) : 16373SwissProt (Human): P78415SwissProt (Mouse) : P81067Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of IRX3 expression in Hela (A), A549 (B) whole cell lysates.
• 
  Immunohistochemical analysis of IRX3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.