Description: Rabbit polyclonal antibody to IL-1F5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-1F5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: IL36RN Alternative Names: FIL1D; IL1F5; IL1HY1; IL1L1; IL1RP3; Interleukin-36 receptor antagonist protein; FIL1 delta; IL-1-related protein 3; IL-1RP3; Interleukin-1 HY1; IL-1HY1; Interleukin-1 delta; IL-1 delta; Interleukin-1 family member 5; IL-1F5; Interleukin-1 receptor antagonist homolog 1; IL-1ra homolog 1; Interleukin-1-like protein 1; IL-1L1Entrez Gene (Human): 26525Entrez Gene (Mouse) : 54450SwissProt (Human): Q9UBH0SwissProt (Mouse) : Q9QYY1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IL-1F5 expression in HEK293T (A), H9C2 (B) whole cell lysates.
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Immunohistochemical analysis of IL-1F5 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.