Description: Rabbit polyclonal antibody to GLURD2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GLURD2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: GRID2 Alternative Names: GLURD2; Glutamate receptor ionotropic, delta-2; GluD2; GluR delta-2 subunitEntrez Gene (Human): 2895Entrez Gene (Mouse) : 14804Entrez Gene (Rat) : 79220SwissProt (Human): O43424SwissProt (Mouse) : Q61625SwissProt (Rat) : Q63226Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GLURD2 expression in zebrafish (A) whole cell lysates.
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Immunofluorescent analysis of GLURD2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Neto2 interacts with the scaffolding protein GRIP and regulates synaptic abundance of kainate receptors