Description: Rabbit polyclonal antibody to GNL3L Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GNL3L. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: GNL3L Alternative Names: Guanine nucleotide-binding protein-like 3-like proteinEntrez Gene (Human): 54552Entrez Gene (Mouse) : 237107SwissProt (Human): Q9NVN8SwissProt (Mouse) : Q6PGG6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GNL3L expression in H1792 (A), MCF7 (B) whole cell lysates.
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Immunohistochemical analysis of GNL3L staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.