Description: Rabbit polyclonal antibody to GATA1 (Phospho-S310) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S310 of human GATA1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: GATA1 Alternative Names: ERYF1; GF1; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding proteinEntrez Gene (Human): 2623Entrez Gene (Mouse) : 14460Entrez Gene (Rat) : 100911167; 25172SwissProt (Human): P15976SwissProt (Mouse) : P17679SwissProt (Rat) : P43429Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of GATA1 (Phospho-S310) expression in K562 (A) whole cell lysates.
-
Immunofluorescent analysis of GATA1 (Phospho-S310) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).