Description: Rabbit polyclonal antibody to Frizzled 1/2/7 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Frizzled 1/2/7. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: FZD1; FZD2; FZD7 Alternative Names: Frizzled-7; Fz-7; hFz7; FzE3Entrez Gene (Human): 8324Entrez Gene (Mouse) : 14369SwissProt (Human): Q9UP38; Q14332; O75084SwissProt (Mouse) : Q61090Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Frizzled 1/2/7 expression in Hela (A), mouse heart (B), rat heart (C), rat kidney (D) whole cell lysates.
-
Immunofluorescent analysis of Frizzled 1/2/7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.