Description: Rabbit polyclonal antibody to Frizzled 4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Frizzled 4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: FZD4 Alternative Names: Frizzled-4; Fz-4; hFz4; FzE4; CD344Entrez Gene (Human): 8322Entrez Gene (Mouse) : 14366Entrez Gene (Rat) : 64558SwissProt (Human): Q9ULV1SwissProt (Mouse) : Q61088SwissProt (Rat) : Q9QZH0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Frizzled 4 expression in A549 (A), Hela (B), CT26 (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of Frizzled 4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.